Resumo

FRESNEDA-DISOTUAR, Anniet et al. Molecular characterization of a Cuban isolation of canine parvovirus. Vaccimonitor [online]. 2015, vol.24, n.3, pp. 0-0. ISSN 1025-0298.

The type 2 canine parvovirus (CPV-2) is the causal agent of an infected-contagious disease that produces a hemorrhagic and acute gastroenteritis that affects young canines. The CPV-2 is a virus with a small, naked DNA genome, very resistant to the environmental conditions that emerged and expanded fast at the end of the 70´s decade. In the 1980, two antigenic variants of CPV-2 emerged almost simultaneously. They were called CPV-2a and CPV-2b. In the 2000, a new antigenic variant was detected. It was called CPV-2c, and was frequently reported in canine communities in some countries worldwide. The objective of this work is to characterize a Cuban isolation of canine parvovirus attenuated and adapted to cellular cultivation. The genetic material was amplified by polymerase chain reaction and sequencing later. The obtained sequences were analyzed and compared with sequences of well-known strains placed in the data base where it was evidenced that the Cuban isolation was of CPV-2 type.

Palavras-chave : canine parvovirus; molecular characterization; PCR.

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