Resumo

LASTRA VALDES, Humberto; RODRIGUEZ LEYES, Eduardo; PONCE DE LEON REGO, Heidy  e  GONZALEZ SANABIA, María Lidia. Método analítico para la cuantificación de taninos en el extracto acuoso de romerillo. Rev Cubana Plant Med [online]. 2000, vol.5, n.1, pp. 17-22. ISSN 1028-4796.

Authors developed an analytical method to quantification of tannins in aqueous extract from Bidens pilosa L. (romerillo) using visible-ultraviolet spectrophotometry, where samples are readed at 700 nm, and its absorbance is referred to tanic acid. Calibration curve for rank of concentrations, included between 4 and 16 ppm, was linear both for reference chemicals (tanic acid) and for aqueous extract from Bidens pilosa L. (romerillo), with correlation coeficients above 0,9900 in two cases; furthermore, statistical tests of interception and slope weren´t significant. Precision given as repeatibility was satisfactory with a variation coefficient (VC) equal to 2,30 % for 12 assays, whereas repetibility fulfills the internationally stablished criteria since variation coeficient was of 2,94 %. Recovery was superior to 99 % in studied concentration ranks and fulfils the "G" Cochran´s and "T" Student´s tests. Method was selective, linear, precise, and accurate.

Palavras-chave : PLANTS, MEDICINAL; MEDICINE, TRADITIONAL; TANNINS; SPECTROPHOTOMETRY, ULTRAVIOLET.

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