Introduction:

Prevention and control of hepatitis B in Cuba has been possible due to introduction of Cuban vaccine Heberbiovac HB, in the National Program of Immunization (NPI). Actually, our health system has the challenge to achieve the elimination of hepatitis B. Objective: To evaluate the prevalence of infection (HBsAg, DNA HBV) and protection (antibodies anti-HBsAg) markers of hepatitis B virus, in blood donors and a vulnerable group, and identify the influence of the immunogenetics of the Cuban population in humoral response to Heberbiovac HB vaccine.

Methods:

Hepatitis B virus markers for infection and protection were measured in blood donors and patients under hemodialysis treatment, using immunoenzymatic and molecular assays. Also, nucleotide sequencing was used to determine single nucleotide polymorphisms (SNP) of interleukin 12 (IL-12), IL-12A G>A e IL-12B A>C, in individuals boosted with a single dose of Heberbiovac HB.

Results:

HBsAg prevalence was low in Cuban blood donors, and zero in donors born after the implementation of HBV vaccination in NPI. In hemodialysis patients, HBsAg prevalence was 5,95%, and 89,47% of them had protector levels of anti-HBsAg. In individuals with SNP IL-12 combinations IL-12A/IL-12B; GG/AC and GG/AA hiperprotectors levels of anti-HBsAg were identified, post-vaccination, as opposed to the AA/AA combination. Conclusions: The findings show the immunogenicity of Heberbiovac HB and confirm the efficacy of the Cuban vaccination program. It was demonstrated for the first time that IL-12 SNP could impact the level of anti-HBsAg antibodies after immunization in the Cuban population.

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