Resumo

VALDES DIEZ, Lilliam et al. Variantes del método de Berthelot. Rev Cubana Farm [online]. 1997, vol.31, n.2, pp. 96-101. ISSN 1561-2988.

Three different methods for the determination of serum urea were assayed, based on the Berthelot reaction using phenol, thymol, and sodium salicylate, and were compared with the method of diacetyl monoxima with the aim of selecting the best and more adequate. Lack of precision, non-accuracy, and lineality of each of them were also evaluated. In all assays performed a lineality from 4.9950 to 24.9750 mmol/L of urea was obtained; when assessing imprecision the values of variation coeficient were found to be lower than 7 and when studying lack of accuracy values of the variation coeficient higher than 0.98 were observed in all cases.

Palavras-chave : COLORIMETRY [methods]; REAGENT KITS, DIAGNOSTIC; UREA [blood]; CHEMISTRY, CLINICAL; PHENOLS; THYMOL; SODIUM HYPOCHLORITE; CALIBRATION; REFERENCE STANDARDS.