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Revista Cubana de Farmacia
versão On-line ISSN 1561-2988
Resumo
GUTIERREZ GAITEN, Yamilet Irene; MIRANDA MARTINEZ, Migdalia; VARONA TORRES, Noel e RODRIGUEZ, Aida Tania. Validación de 2 métodos espectrofotométricos para la cuantificación de taninos y flavonoides (quercetina) en Psidium guajaba, L. Rev Cubana Farm [online]. 2000, vol.34, n.1, pp. 50-55. ISSN 1561-2988.
We validated two spectrometric methods that facilitate to quantify tannins and flavonoids in a raw drug from Psidium guajaba L. Since literature does not provide official methods for quantifying such compounds from this plant. It was determined that both methods (tungsten-molybdic-phosphoric method for tannins by Miranda M., and spectrophotometric method for total flavonoids expressed as quercentin by Kostennikova Z as modified by Méndez G.) meet all the proposed validating requirement for a quality chemical control of the species (linearity, precision (repeatability and reproducibility) accuracy and specificity).
Palavras-chave : SPECTOPHOTOMETRY [methods]; TANNINS [analysis]; QUERCETIN [analysis]; PLANT LEAVES.