Resumo

LAGO, Guillermo; COTO, Gabriel  e  ORUNA, Loida. Aislamiento de polisacáridos a partir de cordones umbilicales humanos. Rev Cubana Farm [online]. 2002, vol.36, n.3, pp. 182-188. ISSN 1561-2988.

The polysaccharides obtained from human umbilical cords by a modification of Danishevsky and Bella’s technique were isolated and characterized. The cords were treated with a sodium chloride solution under controlled temperature and agitation conditions. The polysaccharides were precipitated by adding a disolution of cetyltrimethylammonium 1 %. The sediment obtained in a sodium chloride solution 0.4 mol/L was resuspended, eliminating some impurities that were not soluble at this ionic force by centrifugation. The product of interest was precipitated by adding ethanol to the supernadant and it was characterized by chemical methods. This product may be used as a raw material to obtain a healing gel in the Medicopharmaceutical Industry.

Palavras-chave : POLYSACCHARIDES [isolation & purification]; UMBILICAL CORD; IMMUNOENZYME TECHNIQUES; AMMONIUM SULFATE.