SciELO - Scientific Electronic Library Online

 
vol.39 número2Validación de los métodos analíticos para el control de la calidad y el estudio de estabilidad del colirio de idoxiuridina 0,1 %Establecimiento de un material de referencia para interferón gamma humano recombinante índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Revista Cubana de Farmacia

versão On-line ISSN 1561-2988

Resumo

VEGA, Maribel et al. Establecimiento de un material de referencia de trabajo para interleucina-2 recombinante. Rev Cubana Farm [online]. 2005, vol.39, n.2, pp. 1-1. ISSN 1561-2988.

Establishment of a working reference material for recombinant interleukin-2 Interleukin-2 is a human glycoprotein with 133 aminoacids that is crucial for attaining an effective immunological reaction. In the Center of Genetic Engineering and Biotechnology this protein has been obtained as a recombinant product from Eschericia coli bacteria. For the quality control of every batch produced, it was necessary to establish a working reference material. In this paper we describe the steps taken to obtain a candidate batch and the preparation of the working material as such. It was proved that the working pattern obtained.is homogenous for its use in   the analytical techniques of quality control of interleukin-2. A loss of activity lower than an annual 5 %   was predicted by an accelerated stability study. Adequate values were attained for the purity of the reference material, evaluated by electrophoresis, and for the biological activity that was established with traceability to the international reference material for this product.

Palavras-chave : Reference material; interleukin-2; working reference material; patterns; standard.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License