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Revista Cubana de Farmacia
versión impresa ISSN 0034-7515versión On-line ISSN 1561-2988
Resumen
DIAZ, Miriam et al. Purificación de antígeno de Candida albicans para pruebas cutáneas: estudio preliminar. Rev Cubana Farm [online]. 1996, vol.30, n.1. ISSN 0034-7515.
The assessment of cellular immunity by means of the response produced by T-lymphocytes stimulated by the presence of a determined antigen, is of great interest in the treatment of several infectious diseases during the course of burns, lesions, traumas, etc., and due to this it is very important to obtain purified antigens for their usage in the skin tests used in such assessment. The authors developed a purification method to obtain one of these antigens, from Candida albicans. They carried out modifications on the method described by Buckley et al., and found that the methodology was adequate and reproducible, since the antigen obtained had similar characteristics to those of the reference antigen.
Palabras clave : ANTIGENS [isolation and purification]; CANDIDA ALBICANS [isolation and purification]; IMMUNITY, CELLULAR; T-LYMPHOCYTES; SKIN TESTS.