Abstract

ROJAS MARTINEZ, Roberto; SANCHEZ SANCINENAS, Marlene  and  MORON RODRIGUEZ, Francisco. Actividad biológica de la prostaglandina F₂ alfa y del metil carboprost en el fundus gástrico aislado de rata. Rev Cubana Farm [online]. 1997, vol.31, n.2, pp.113-118. ISSN 0034-7515.

The contractil potency of a sample of methylic ester of 15 methyl prostaglandin F₂ alpha (methyl carboprost) (I) produced by the Cuban Pharmaceutical Industry, was compared to a commercial sample of prostaglandin F₂ alpha (II) from a Chinoin firm through the "three point" assay. The duration of the contraction induced by such samples was also compared using the analysis of linear regression of the relaxation observed in function of time after the supression of contact between the drug and the effector organ. The gastric fundus isolated from a rat was used as the effector organ. The contractil activity was recorded by strength-displacement transducers. Sample I showed a relative potency of 164.0 % with respect to sample II, with confidence limits of 111.2 and 247.8 % (P = 0.95; N=7) and it also showed a more prolonged effect in relation to sample II. In the strips contracted by sample I the elapsed should have been 6 min and 46 sec after washing, for the contraction to be reduced to a half of the minimun response observed (N=7), while in strips contracted by sample II the time elapsed was 1 min and 48 sec (N=8).

Keywords : DINOPROST [pharmacology]; CARBOPROST [pharmacology]; GASTRIC FUNDUS [drug effects]; MUSCLE CONTRACTION [drug effects]; BIOLOGICAL ASSAY; RATS; INBRED STRAINS.