Revista Cubana de Farmacia
On-line version ISSN 1561-2988
SUAREZ PEREZ, Yania; GARCIA PULPEIRO, Oscar and CUETO LEON, Marisel. Design and validation of a new method for estimation of water-soluble reducers associated with chitin. Rev Cubana Farm [online]. 2005, vol.39, n.3, pp. 0-0. ISSN 1561-2988.
For the first time, a spectrophotometric method to estimate water-soluble reducers associated with raw material chitin was developed. For the purpose of adjusting the method, glucosamine as a reference substance was used. The estimations were made at 330 nm that was the wavelength of maximum absorption. The selected optimal heating time was 5 min; the selectivity of analytical response was checked. The Lambert-Beer law was demonstrated in the 100-300 mg/mL interval. An aqueous extract from raw material chitin was obtained and then processed to eliminate possible interference and be used as a control for the method validation. The method was linear, exact and accurate in the studied interval. The detection limit was 25,21 mg/mL and the quantification limit was 65,45 mg/mL.
Keywords : Chitin; stability; hydrolysis products; glucosamine; spectrophotometry.