Resumo

DIEGUEZ CASTRO, R. Rosario; MUNOZ TRUJILLO, Yanin  e  CASTINEIRA DIAZ, Mirta. Western Blot technique applied to VA MENCOG BC^® antimeningococcal vaccine. Rev Cubana Farm [online]. 2009, vol.43, n.3, pp. 0-0. ISSN 1561-2988.

ABSTRACT Western Blot technique applied to VA MENGOC BC^® antimeningococcal vaccine was developed and validated and produced in "Carlos J. Finlay" Institute to demonstrate the identity criterion. Using this technique it was possible to identify antigenic proteins type P₁, P₃, P₅ 70 and 80 K present in the vesicle of external membrane and final vaccine, thus, we used the antimeningococcal gamma. Parameters developed in validation of this technique included: specificity, detection limit, repetition, average accuracy, reproduction, and strength, identity technique fulfilled with abovementioned parameters, considering like validated

Palavras-chave : Western Blot Technique; Western blot validation; identity; VA MENGOC BC^® antimeningococcal vaccine.

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