Resumo

DIEGUEZ CASTRO, R. Rosario; MUNOZ TRUJILLO, Yanin  e  CASTINEIRA DIAZ, Mirta. Western Blot technique as an identity criterion for Men B antimeningococcal vaccine. Rev Cubana Farm [online]. 2009, vol.43, n.3. ISSN 0034-7515.

Western Blot technique was developed and validated, applied to Men B meningococcal vaccine produced in "Carlos J, Finlay" Institute to demonstrate an identity criterion. In study of antigenic proteins of the vaccine, we used P1.15 y P1.4 in vesicle of external membrane, monogranels, and end product to identify the monoclonal antibodies specific of these proteins. Parameters developed in technique validation included: specificity, detection limit, repetition, average accuracy, reproduction, and strength. Method fulfilled with specified parameters, thus considering its validation.

Palavras-chave : Western blot technique; Men B antimeningococcal vaccine; identity; Western blot validation.

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