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vol.43 número4Evaluación de propiedades físico-químicas de tinturas de Quassia amara y Maytenus ilicifoliaEstatinas, una nueva opcion terapéutica en diversos tipos de enfermedades índice de autoresíndice de materiabúsqueda de artículos
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Revista Cubana de Farmacia

versión impresa ISSN 0034-7515versión On-line ISSN 1561-2988

Resumen

SIERES PEDRAJA, Eneyda  y  NIETO ACOSTA, Olga María. Stability by thin-layer chromatography from Quassia amara and Maytenus ilicifolia tinctures. Rev Cubana Farm [online]. 2009, vol.43, n.4, pp.93-101. ISSN 0034-7515.

In past years, a research field with boom is that related to phytotherapeutical products, being necessary an integral research for its latter utilization. Present paper describes a thin layer chromatography (TLC) method to assess the stability of tinctures obtained from a percolation process of two Brazilian plants: Quassia amara and Maytenus ilicifolia. Study took into account three storage temperatures: refrigeration, room-temperature and at 40 ºC during 90 days. Chomatographic conditions were selected, detection limit was determined, and method selectivity was demonstrated where the extract undergoes four stress-conditions. As a mobile phase we used n-butanol-acetic acid-water (4:1:5), and as UV at a l of 366 nm. The better results are obtained when analyzed extract is stored in refrigeration during study-time. Under stress-conditions only it is possible to obtain favorable results under UV. Detection limit is that corresponding to 7 µL.

Palabras clave : Stability; thin layer chromatography; detection limit; stress conditions; storage.

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