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Revista Cubana de Farmacia

versión impresa ISSN 0034-7515versión On-line ISSN 1561-2988

Resumen

SANCHEZ PERRERIA, Luz María; MANCEBO DORVIGNY, Betty; FAURE GARCIA, Roberto  y  TRAVIESO, María del Carmen. Validation of the technique to assessment of tablets Catechin from Rhizophora mangle L. by HPLC. Rev Cubana Farm [online]. 2010, vol.44, n.1, pp.58-70. ISSN 0034-7515.

Authors made the high-performance liquid chromatography method validation to the quantitative assessment of cathechin as a marker substance in tablets obtained from the bark of Rhizophora mangle L. dry extract used in gastroduodenal ulcers treatment. Considering that this method as such is classified to quantitative assessment of the major compound or active ingredient in formulae or raw material, the following parameters were assessed : linearity, accuracy, sensitivity and precision expressed in its two ways: repetition and intermediate precision. Results obtained showed that this method is reliable allowing the compound assessment in presence of other substances, including excipients and auxilliary substances and to detect the presence of degradation products. Also, the statistical processing of results evidenced the linearity, precision, sensitivity and accuracy of this method.

Palabras clave : Rhizophora mangle L; ethyl-acetate fraction of polyphenols; cathechin; high-performance liquid chromatography; quality control; stability study.

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