SciELO - Scientific Electronic Library Online

 
vol.47 número3Caracterización espectroscópica del D-003 obtenido de la cera de caña de azúcar (Saccharum officinarum L.)La carrera de Farmacia en Cuba desde el curso 1921-1922 hasta el 1924-1925 índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Revista Cubana de Farmacia

versión impresa ISSN 0034-7515

Resumen

PEREZ GUERRA, Yohani et al. Effect in vitro of D-003 on 5-lipooxygenase (5-LOX) enzyme activity. Rev Cubana Farm [online]. 2013, vol.47, n.3, pp.400-407. ISSN 0034-7515.

Introduction: D-003, a mix of higher primary aliphatic acids purified from sugarcane, inhibits cholesterol synthesis. Recent studies have demonstrated that D-003 is effective in experimental models of osteoarthritis and inhibits the enzymatic activities of COX1 and COX2, mainly that of COX1, without causing gastrotoxicity. It has been mentioned that the dual inhibitors of COX and 5-LOX enzymes present with anti-inflammatory effects and no gastrotoxicity or even they can have gastroprotective actions. According to this background, D-003 could be a dual inhibitor of COX and 5-LOX enzymes. Objective: to study the effects of D-003 on the enzymatic activity of 5-LOX in vitro, by using the cytosolic fraction of polymorphonuclear leukocytes of rats. Methods: the following testing conditions were used: cytosolic fraction (50 µg of protein) dissolved into 0,2 mol/L borate buffer solution (pH 9) and linoleic acid (7,8-250 mmol/L) as substrate. Parallel samples were incubated with Tween-20/H2O (2 %) only (vehicle), D-003 (0,6-6 000 µg/mL) or green-lipped mussel (Perna canaliculus) extract (50 µg/mL) (reference substance). The enzymatic activity, evaluated by the conjugated diene formation, was assessed by the absorbance changes at 234 nm (5-LOX) measured in a UV-visible spectrophotometer. Results: by adding D-003, produced a dose dependent (r= 0,975; p< 0,05) (IC50= 23,06 µg/mL) in vitro inhibition of 5-LOX enzyme activity. The size of the inhibition was moderate, since the maximal inhibition, achieved from 1 250 µg/mL) on was only 30 %. Conclusions: this study demonstrates that D-003 may inhibit in vitro the 5-LOX enzymatic activity, but in a moderate way.

Palabras clave : higher primary aliphatic acids; D-003; 5-LOX inhibition; lipooxygenases.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons