Abstract

VARONA TORRES, Noel et al. Validation of a high performance liquid chromatography method for quantitation of L-proline in 20 % tincture from Murraya paniculata L. Jack. Rev Cubana Farm [online]. 2014, vol.48, n.2, pp.296-306. ISSN 0034-7515.

Introduction: the search for analytical methods that may monitor the quality of drugs is an issue of great interest in the pharmaceutical field, even more if they are directed to studying chemical markers of medicinal plants, their extracts and phytomedicines. Objective: to validate a high-resolution liquid chromatography (HPLC) method for the quantitative determination of the L-proline amino acid as a marker substance in Murraya paniculata L. Jack tincture. Methods: in the HPLC, the separation was performed on a C-18 (UP5ODB-150/046) column, with a mixture of phosphate buffer solution, pH adjusted to 2.4 and acetonitrile (70:30 v/v) used as mobile phase, the flow rate was 0.6 mL/min, isocratic mode with UV detection set at 440 nm. The injection volume of the sample was 20 µL. The method was validated according to category I, following international requirements. Results: the calibration curve was linear over the concentration range tested (30-375 mg/mL), good precision was observed with a variation coefficient less than 2 %. Recovery values were within the limits for chromatographic methods (98-102 %). The method was specific since there was no-interference by additional peaks in the elution zone of the compound in question (L-proline). Conclusions: the HPLC analytical method, validated for the quantification of L-proline amino acid in M. paniculata tincture, proved to be linear, precise, accurate and specific under the study conditions.

Keywords : Murraya paniculata L; L-proline; chemical marker; high performance liquid chromatography (HPLC); quality control.

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