Abstract

HERNANDEZ FALCON, Daylín; FERNANDEZ GARCIA, Asela  and  LEDEA LOZANO, Ernesto. Validation of analytical method for dopamine determination by high-performance liquid chromatography. Rev Cubana Farm [online]. 2014, vol.48, n.3, pp.371-381. ISSN 0034-7515.

Introduction: dopamine is an endogenous hormone in the catecholamine group, which is used to simulate the sympathetic nervous system action and to raise the heart rate and the blood pressure. However, this drug has high genotoxicity and is associated with the treatment of diseases such as schizophrenia and Parkinson's disease. For this reason, the determination of dopamine in wastewaters coming from its production has been of great interest. Objective: to validate a high performance liquid chromatography method for quantification of dopamine present in aqueous solutions. Methods: the validation used a RP-18 250 x 4.6 mm 5 µm column, mobile phase: 1 % NaH₂PO₄ /CH₃OH (90/10), 1.0 mL/min flow rate and a visible ultraviolet detector at 280 nm. Detection limits and quantitation were estimated in addition to evaluating the drug stability under the analysis conditions. Results: documented evidence showed that the method proved to be linear (r= 0.999 and r²= 0.998), accurate and precise in the range of 1 x 10^-4 mol/L to 2 x 10^-3 mol/L. This method was also selective against potential degradation products from ozonization. Conclusions: the studied method was linear, precise and accurate in the range of tested concentrations, allowing the dopamine determination in aqueous solutions.

Keywords : dopamine; High Performance Liquid Chromatography; validation.

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