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Revista Cubana de Medicina
versão impressa ISSN 0034-7523versão On-line ISSN 1561-302X
Resumo
MANDADO PEREZ, Sara et al. Diagnóstico morfológico de Helicobacter pylori mediante citología gástrica por cepillado. Rev cubana med [online]. 2003, vol.42, n.1, pp.27-33. ISSN 0034-7523.
It is known that in Cuba the gastric brush citology has not been used for the morphological diagnosis of Helicobacter pylori. It was performed by endoscopy to 625 adult patients , 585 with chronic gastritis and 40 with stomach ulcer, at the Institute of Gastroenterology. The samples were fixed in alcohol 95 %. Papanicolaous stains, hematoxylin and eosin , Grams stain, safranin and methylene blue were tested. The samples were observed on a previously calibrated optical microscope. H. pylori was diagnosed in 310 chronic gastritis and in 28 ulcers, 2 of them were malignant. H. heilmannii was diagnosed in a case with stomach ulcer negative to H. pylori. Methylene blue 2 % was selected for staining.
Palavras-chave : HELICOBACTER PYLORI [citology]; HELICOBACTER PYLORI [isolation & purification]; GASTRITIS [diagnosis]; STOMACH ULCER [diagnosis]; ENDOSCOPY, DIGESTIVE SYSTEM; STAINING [methods].