SciELO - Scientific Electronic Library Online

 
vol.40 issue1Consideracions on primitive cesarean rateVideolaparoscopic staging in endometrial adenocarcinoma author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Revista Cubana de Obstetricia y Ginecología

Print version ISSN 0138-600X

Abstract

RIVERO FIGUEROA, Dianelys et al. Detection of Chlamydia trachomatis in endocervical swab using rapid test and two reaction techniques polymerase chain. Rev Cubana Obstet Ginecol [online]. 2014, vol.40, n.1, pp.48-57. ISSN 0138-600X.

Introduction: Chlamydia trachomatis is the leading bacterial agent that causes sexually transmitted infections. Objective: to detect the presence of C. trachomatis using a rapid test and compare it with the chain reaction (PCR). Methods: 50 endocervical exudates taken from symptomatic women were processed in Diez de October municipality. The samples were applied the Chlamy-check-1 test, a PCR assay of the cryptic plasmid gene and a real-time PCR (RT-PCR) of major outer membrane protein (MOMP) of C. trachomatis which was used as reference. Sensitivity, specificity, positive (PPV) and negative (NPV) predictive value were calculated. Results: 44 samples were positive by the rapid test, whereas only three samples (6%) amplified by cryptic plasmid PCR. Applying RT-PCR, 4 samples (8%) were confirmed as positive, 3 samples matched with three diagnostic methods. In assessing the Chlamy-check-1 versus the reference test, 100% of sensitivity was observed, while the specificity was 13%> Also PPV was 9.1% and NPV was 100%. On the contrary, the cryptic plasmid PCR had 75 and 100% of sensitivity and specificity respectively, 100% PPV and 97.9% NPV. Conclusions: the difference was obtained between the percentages of positivity detected with both the rapid test, and CPR techniques. The low specificity of the rapid test indicates the need for further studies to evaluate this diagnostic kit.

Keywords : Chlamydia trachomatis; Chlamydia check-1; chain reaction polymerase; cryptic plasmid; major outer membrane protein (MOMP).

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License