Resumen

RIVERO FIGUEROA, Dianelys et al. Detection of Chlamydia trachomatis in endocervical swab using rapid test and two reaction techniques polymerase chain. Rev Cubana Obstet Ginecol [online]. 2014, vol.40, n.1, pp.48-57. ISSN 0138-600X.

Introduction: Chlamydia trachomatis is the leading bacterial agent that causes sexually transmitted infections. Objective: to detect the presence of C. trachomatis using a rapid test and compare it with the chain reaction (PCR). Methods: 50 endocervical exudates taken from symptomatic women were processed in Diez de October municipality. The samples were applied the Chlamy-check-1 test, a PCR assay of the cryptic plasmid gene and a real-time PCR (RT-PCR) of major outer membrane protein (MOMP) of C. trachomatis which was used as reference. Sensitivity, specificity, positive (PPV) and negative (NPV) predictive value were calculated. Results: 44 samples were positive by the rapid test, whereas only three samples (6%) amplified by cryptic plasmid PCR. Applying RT-PCR, 4 samples (8%) were confirmed as positive, 3 samples matched with three diagnostic methods. In assessing the Chlamy-check-1 versus the reference test, 100% of sensitivity was observed, while the specificity was 13%> Also PPV was 9.1% and NPV was 100%. On the contrary, the cryptic plasmid PCR had 75 and 100% of sensitivity and specificity respectively, 100% PPV and 97.9% NPV. Conclusions: the difference was obtained between the percentages of positivity detected with both the rapid test, and CPR techniques. The low specificity of the rapid test indicates the need for further studies to evaluate this diagnostic kit.

Palabras clave : Chlamydia trachomatis; Chlamydia check-1; chain reaction polymerase; cryptic plasmid; major outer membrane protein (MOMP).

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