Abstract

CORONA, Belkis  and  MARTINEZ, Siomara. DETECTION OF Anaplasma marginale IN BOVINE, USING THE msp5 GENE AMPLIFICATION BY PCR. Rev Salud Anim. [online]. 2011, vol.33, n.1, pp.24-31. ISSN 0253-570X.

Anaplasma marginale is a rickettsia of Ehrlichias genogroup II. It parasites mature erythrocytes in bovines. There is not still an effective control method against the disease; thus it is of great importance to develop an immunogene able to prevent infection by this pathogen. There is also a need of having more sensitive diagnostic techniques which allow the detection of carrier animals in order to be used in cattle international movement towards areas free of the disease; as well as to know the prevalence of the disease in tropical and subtropical regions. Gene msp5 is represented in the genome as a simple copy; it is highly preserved among all Anaplasma species and all A. marginale strains; thus it is an important candidate for being used in diagnostic. In this paper, the amplification by PCR of such gene was carried out for the detection of Anaplasma marginale in 113 animals without clinical symptoms, from which 96 were positive to this microorganism, being the assay highly sensitive and specific.

Keywords : Anaplasma marginale; diagnosis; PCR; msp5 gene.

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