Resumen

CORONA, Belkis et al. DETECTION BY PCR Anaplasma marginale IN BUFFALOES OF THE WESTERN REGION OF CUBA. Rev Salud Anim. [online]. 2012, vol.34, n.1, pp.11-18. ISSN 0253-570X.

The buffalo species was recently introduced in Cuba, showing excellent productive and reproductive performance. Their health situation has been little studied, although their natural resistance to various diseases reported in the country, including haemoparasitosis has been recognized. Buffaloes are also susceptible to infection with Anaplasma marginale, but hardly present clinical anaplasmosis, remaining asymptomatic carriers, which facilitates transmission to cattle. This study is aimed at determining the presence of A. marginale in buffaloes of the western region of Cuba. For this purpose, an nPCR test, based on msp5 gene amplification of this hemoparasites, was developep. Fifty-two DNA samples, extracted from animal blood, randomly selected from 6 farms in western Cuba, which underwent blood smear were analized. From the total samples analyzed, 47 (90.4%) were positive by nPCR, visualizing in all cases a band of 345 bp, while only 5 animals (9.6%) were positive in blood smear. One of the fragment amplified was sequenced and the sequence obtained was deposited in GenBank under Accession Number JF270381, showing a high percentage of identity with Anaplasma marginale deposited sequences in different regions of the world.

Palabras clave : Anaplasma marginale; nPCR; msp5.

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