Abstract

HERNANDEZ, P.J.E et al. Post-thaw acrosomal viability and reaction in sperm obtained from equine epididymis tail. Rev Salud Anim. [online]. 2012, vol.34, n.2, pp.84-88. ISSN 0253-570X.

In order to evaluate the post-thaw effect on acrosomal viability and reaction of sperm collected from equine epididymis tail, 62 testes were collected and transported at 4°C. Epididymis was dissected to perform the retrograde flushing of the epididymis tail , using 10 mL of a diluent based on skim milk (INRA 82). The diluent obtained with the spermatic content was recovered in graduated collecting tubes and 1mL of each sample was taken to be evaluated. For evaluations, the samples having a minimum of 30% of progressive motility were selected. The evaluations were on: percentage of live sperm, normal morphology, sperm concentration, percentage of live sperm without acrosomal reaction (VsRA) and live with acrosomal reaction (VcRA), evaluating this indicator with FITC-PNA staining and propidium iodide (PI). Averages of 53.2%, 72.7%, 82.9%, 507.3X106, 65.3% and 6.0% were respectively obtained. Subsequently, samples were packed into 0.5 ml straws at a 100x106 concentration, frozen in liquid nitrogen and stored for 8 days. Thawing was performed at 38°C for 37 seconds, resulting for sperm motility, percentage of live sperm, normal morphology and VcRA VsRA the following values: 26.3%, 60.9%, 73.8%, 43.4% and 14.2%, respectively. Although all studied sperm quality values were affected by cryopreservation (P <0.05), a 43.4% of cells was recovered with characteristics making them suitable for use in biotechnological applications of assisted reproduction.

Keywords : cryopreservation; viability; acrosomal reaction; FITC-PNA/IP; sperm; epididymis; equine.

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