Abstract

PENA, Joan  and  UFFO, Odalys. Biofilm production of Staphylococcus aureus genotypes isolated from bovine mastitis in Cuba. Rev Salud Anim. [online]. 2013, vol.35, n.3, pp.189-196. ISSN 0253-570X.

Staphylococcus aureus is one of the major causes of bovine mastitis. Biofilm production by intramammary pathogens provides microorganisms a higher capacity to colonize the host tissue and protection against the host immune system and the protective action against antimicrobial agents. In this study, 98 S. aureus isolates were collected from five Cuban provinces; all isolates were tested for slime and biofilm formation, and the genetic background related to the polymorphic X-region of protein A was recorded. A high percent (98%) of Cuban isolates presented some biofilm production. These results suggest that microplate by Crystal Violet staining method for biofilm detection is the election method, since slime methodology by inoculation in Red Congo Medium is not useful for inferring biofilm production, due to a high false negative number of strains found. It was determined that t605 genotype it is associated to a high biofilm production, then a greater pathogenic potential should be expected.

Keywords : biofilm; slime; Staphylococcus aureus; bovine mastitis; Spa genotype.

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