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Revista de Salud Animal
versión impresa ISSN 0253-570Xversión On-line ISSN 2224-4700
Resumen
AGUILAR-BULTET, Lisandra y FALQUET, Laurent. Sequencing and de novo assembly of bacterial genomes: an approach to study new. Rev Salud Anim. [online]. 2015, vol.37, n.2, pp.125-132. ISSN 0253-570X.
Next Generation Sequencing technologies have revolutionized the field of genomics allowing sequencing of a large number of genomes in a short period of time. The genome provides, in principle, the complete catalogue of genes which can be expressed by a microorganism. But the interpretation of this information, at each level (from the huge volume of raw data originated by the sequencer to the thousands of genes identified in parallel associated with various functions), is a major challenge from the computational point of view. De novo assembly is one of the approaches used to reconstruct genomes, particularly bacterial genomes. In this paper the main trends regarding sequencing, and specifically the de novo assembly, are reviewed.
Palabras clave : genome sequencing; next generation sequencing; de novo assembly.