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Revista de Salud Animal

versión impresa ISSN 0253-570Xversión On-line ISSN 2224-4700

Resumen

VERA-MEJIA, Ronald et al. Lactobacillus plantarum strains with probiotic potentials isolated from creole pigs. Rev Salud Anim. [online]. 2018, vol.40, n.2, e04. ISSN 0253-570X.

The objective of the present work was to characterize, by means of in vitro tests, two strains of Lactobacillus plantarum (22 CML and 41 CML), isolated from the caecum mucosa of Creole pigs. Their probiotic activity was evaluated by the growth stability tests at different temperatures (30, 37 and 45°C) and pH (3.4; 4; 5.4; 6.7; 7.5.), as well as the acidification capacity of the culture medium, tolerance to artificial gastric juice and bile salts. Adherence tests, such as autoaggregation, hydrophobicity and microbial antagonism, were performed in four pathogenic strains. The strains grew in all the conditions evaluated, showing probiotic potentialities for their resistance to the treatment with bile salts until 24 hours. Microbial growth was observed from 4.20 -5.17 log CFU/ml at 90 minutes with artificial gastric juice. They were self-aggregating and hydrophobic, and inhibited the growth of Escherichia coli, Salmonella sp., Staphylococcus aureus and Listeria monocytogenes. The strain L. plantarum 22 LMC presented the best results as probiotics. The inhibitory substances produced by L. plantarum were quantified as lactic acid (1, 05-2, 8 gL-1) and hydrogen peroxide (0, 20- 0, 28 gl-1). The susceptibility profile to 14 antimicrobials was evaluated, and most of the strains revealed resistance to four of them.

Palabras clave : probiotic; Lactobacillus plantarum; pigs.

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