Resumo

LUGO-MARANTE, Sonia et al. Microbiological and genotypic characterization of Pasteurella mutocida strains associated with the cunicular respiratory syndrome. Rev Salud Anim. [online]. 2019, vol.41, n.1, e02.  Epub 01-Abr-2019. ISSN 0253-570X.

Pasteurella multocida is a Gram-negative bacterium associated with respiratory syndromes in different species of animals such as pigs, birds, rabbits, cats, and dogs. This bacterium presents high intra-species heterogeneity, where it is possible to find different biovars, five capsular serogroups with differences in virulence factors and the expression of pathogenicity, as well as profiles of susceptibility to antibiotics and the capacity for the formation of biofilms. The objective of this work is to characterize of P. multocida strains isolated from rabbits. The microbiological identification, capsular genoserotyping and the detection of genes involved in the virulence factors were carried out. Susceptibility to several families of antibiotics and the ability to produce biofilms in vitro were determined. It was confirmed that the strains belong to the species P. multocida subspecies multocida biotype 1 capsular A serogroup. The strains were positive to the detection of loci gene fragments related to the biogenesis of fimbrias, neurominidase and outer membrane proteins, which constitute virulence factors, specifically for colonization and interaction with the host. The strains revealed multi-resistance and only two of them showed moderate capacity for the production of biofilms. The characterization of these strains clarifies the epidemiological aspects related to the capsular serogroup, which are useful for obtaining vaccine candidates and contribute to the prevention or prophylaxis of infections by this bacterium, thus the present work contributes to the control of this infection in rabbits.

Palavras-chave : biofilms; virulence factors; Pasteurella multocida; antimicrobial resistance; capsular serogroup.

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