Resumo

SOTO-MARTINEZ, Yury Grisel; CASAS-HERNANDEZ, Eduardo; BETANCOURT-RULE, José Miguel  e  FERNANDEZ-REYES, Filiberto. In vitro-fertilized bovine embryos co-cultured with oviductal and cumulus oophorus cells. Rev Salud Anim. [online]. 2019, vol.41, n.1, e01.  Epub 01-Abr-2019. ISSN 0253-570X.

The research was aimed at evaluating the effect of embryos co-cultured with oviductal and cumulus oophorus cells on the development of blastocyst in vitro. Oviducts and ovaries were collected from slaughtered bovine females. Oviductal cells were obtained after a longitudinal incision of the oviduct and cumulus cells were collected from follicular fluid; both cell types were placed for culture in TCM-199 with 20 % Fetal Bovine Serum (FBS). The cumulus-oocyte complexes (COC's) were collected by the aspiration of follicles from 2 to 7 mm diameter and they were placed for maturation in TCM-199 with 10 % FBS. Subsequently, they were fertilized using a concentration of 1x106 spermatozoa/mL. After fertilization, they were divided into three treatments: 1) Control, 2) Co-culture with cumulus cells and 3) Co-culture with oviduct cells. The development was evaluated at 192 hours of in vitro culture, determining the amount of divided embryos, morulas and blastocysts. They were cultured in sequential SOF medium by the treatment: 1) 538 oocytes, 2) 627 oocytes and 3) 478 oocytes. The results were the following: 14 % of blastocysts in the co-culture of cumulus cells, 7 % in the co-culture of oviduct and 4 % in the control, with a significant statistical difference (p<0.05) among the three treatments. It is concluded that the use of somatic cells in conditioned medium can stimulate the blastocyst stage development in vitro to the bovine embryos.

Palavras-chave : embryo development; bovine; in vitro; co-culture.

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