Resumo

SANCHEZ MIRANDA, Lilian; VERA MEJIA, Ronald; AGUERO, Félix  e  VEGA CANIZARES, Ernesto. Biological safety of Lactobacillus plantarum 22 LMC with probiotic potential. Rev Salud Anim. [online]. 2020, vol.42, n.2, e10.  Epub 01-Ago-2020. ISSN 0253-570X.

This work was aimed at evaluating the biological safety of a Lactobacillus plantarum 22 LMC strain with probiotic potential, isolated from the caecum mucosa of Creole pigs. A pre-clinical study was conducted to evaluate the effect of the probiotic candidate in an acute oral single-dose model 1012 CFU mL-1. Female and male Sprague Dawley rats were used divided into a group of five animals of each sex per treatment (3): viable L. plantarum cells (1), heat-inactivated cells of the same microorganism (2) and saline solution as a negative control (3) for 14 days; subsequently proceeded to euthanasia. Pathological and microbiological studies of the main organs were carried out. Some hematological and biochemical indices were determined. No toxic effects attributable to the administration of the probiotic were found; body weight was shown to be characteristic for the species and there were no alterations in organ weight. No macroscopic, histopathological and bacterial translocation variations were observed in the organs evaluated. The hematological and blood biochemical studies showed normal values for the species. It is concluded that the L. plantarum 22 CML strain was shown to be biologically safe at the single evaluated dose.

Palavras-chave : acute oral toxicity; Lactobacillus plantarum; probiotic.

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