versão ISSN 0258-5936
DUENAS HURTADO, Francisco; ALVAREZ GIL, Marta; MOYA LOPEZ, Carlos e MARTINEZ ZUBIAUR, Yamila. Identification of the gene Ty-3, begomovirus resistance in accessions of Solanum lycopersicum L. cultrop [online]. 2011, vol.32, n.2, pp. 136-142. ISSN 0258-5936.
Tomato yellow leaf curl disease is one of the most important problems affecting tomato cultivation in most part of the Cuban growing areas and in other countries. The casual agent is the Tomato yellow leaf curl virus (TYLCV). At the moment several genes related with the resistance have been described. The genes Ty-1, Ty-2 and Ty-3 are those that more they have been used in the plant breeding programs of the species. In Cuba, Vyta is only variety that had Ty-1 gene and other accessions carrying the Ty-2 gene. The amplification of the gene Ty-3 was carried out for PCR and were used the specific primers FLUW-25R and FLUW-25F. In this work the presence of the band related with the resistant pattern of the gene Ty-3 was identified in one that six characterized accessions
Palavras-chave : tomato yellow leaf curl geminivirus; genetic resistance.