Abstract

GRANA SANCHEZ, RENE; SILVA, ELADIO; RUIZ, NANCY  and  PEREZ, MARIA TERESA. Purificación de la proteína de 24 KD natural (P24) del virus de inmunodeficiencia humana tipo-1 (VIH-1) utilizando inmunoafinidad. Rev Cubana Med Trop [online]. 1996, vol.48, n.2, pp.98-101. ISSN 0375-0760.

The use of systems to detect the presence of 24kd protein as well as the levels of antibodies to it, is very useful in the follow-up of HIV-1 infected individuals. To develop these systems it is necessary to have this protein purified. The present paper describes the purification of p24 starting from the semipurified viral flattening on saccaharose gradient, using immunoaffinity chromatography with monoclonal antibodies coupled to Sepharosa 4B activaded with cyanogen bromide. For the characterization of the purified product it was used polyacrylamide gel electrophoresis with silver staining and immunoelectrotransference. As a result, it was obtained p24 which can be used for the development of diagnostic tools and monoclonal antibodies.

Keywords : HIV CORE PROTEIN P24 [isolation & purification]; HIV-1 [isolation & purification]; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS: POLYACRYLAMIDE GEL; FOLLOW-UP STUDIES.