Abstract

GONZALEZ, GREHETE et al. Identificación por ELISA de agentes productores de efecto citopático ligero aislados durante la epidemia de neuropatía en Cuba. 1991. Rev Cubana Med Trop [online]. 1996, vol.48, n.2, pp. 133-135. ISSN 1561-3054.

Using as a reference the strain 44/93 isolated during the neuropathy epidemy in 1991 and characterized as a producer of a light cytopathic effect (L-CPE), it was possible the standardization of an ELISA for the fast identification of other strains with similar effect. The assay consisted in a sandwich-type ELISA where the conditions selected for each reactive (10 mg/mL for the coating antibody, 1 mg/mL for the antigen, and dilution 1/2 000 for the conjugate) allowed to have an adequate discrimination between the antigen and the antigen control for the reference strain used. The evaluation of a panel of reference viral strains and of other L-CPE-producing strains showed a 100 % of coincidence between this method and the isolation in cellular culture. The results obtained permit us to recommed the use of this assay as a more precise alternative to identify these agents.

Keywords : ENZYME-LINKED IMMUNOSORBENT ASSAY; NEURITIS; CUBA.