Abstract

FACHADO, ALBERTO; FERNANDEZ, NEYDA; HERNANDEZ, ERNESTO  and  FONSECA, LISSET. Toxoplasma gondii: caracterización de un anticuerpo monoclonal anti-P30. Rev Cubana Med Trop [online]. 1996, vol.48, n.3, pp.178-183. ISSN 0375-0760.

A specific monoclonal antibody was characterized to Toxoplasma gondii. The hybridoma produced IgG immunoglobulins. The Western Blot analysis showed that the monoclonal antibody was specific for the antigen of an apparent mollecular mass of 30 kd, which was present on the antigen surface. The monoclonal antibody was purified starting from mouse´s ascitic fluid and it was matched with Sepharose 4B. This immunoabsorbent was used to purify the specific parasitary antigen. The monoclonal antibody studied may be useful for those tecniques contributing to the toxoplasmosis diagnosis.

Keywords : TOXOPLASMA [immunology]; ANTIGENS, PROTOZOAN [isolation and purification]; ANTIBODIES, MONOCLONAL [isolation and purification]; HYBRIDOMAS BLOTTING, WESTERN [methods]; ENZYME-LINKED IMMUNOSORBENT ASSAY [methods]; IGG; MEMBRANE PROTEINS..