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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054

Abstract

TORRES, DINORAH; FERNANDEZ, MARIBEL; BRITO, TANIA  and  FINLAY, CARLOS. Ensayo inmunoenzimático en fase sólida para la detección de antígenos de Giardia lamblia . Rev Cubana Med Trop [online]. 1997, vol.49, n.1, pp.52-58. ISSN 0375-0760.

An ELISA sandwich for the detection of Giardia lamblia antigens in human faeces was standardized. 175 samples were studied: 77 positive, 61 negative to cysts and/or trophozoites by direct faeces test, and 19 positive to other parasite different from G. lamblia. The sensitivity of the technique was 94,8 % and the specificity 98,3 %. The method detects an antigen concentration of 31 ng. The procedure is simple, sensitive and specific so, it may be useful for diagnosis and in epidemiological studies.

Keywords : GIARDIA LAMBLIA [immunology]; ANTIGENS, PROTOZOAN; ENZYME-LINKED IMMUNOSORBENT ASSAY [methods]; FAECES [parasitology].

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