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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054

Abstract

PUPO ANTUNEZ, MARITZA et al. Anticuerpos monoclonales (AcM) que reconocen el virus herpes simple (HSV) y su posible aplicación al diagnóstico. Rev Cubana Med Trop [online]. 1997, vol.49, n.3, pp.181-185. ISSN 0375-0760.

It is reported the obtention of monoclonal antibodies, which are capable of recognizing HSV-1 and HSV-2 viruses, by the conventional technology of cellular fusion. The monoclonal antibodies of highest positivity according to the indirect immunofluorescence technique were partially characterized. It was determined the neutralizing capacity of 2 of them (278-F7 and 70-G10), which were used to identify isolations of clinical specimens by indirect immunofluorescence. These monoclonal antibodies recognized HSV-1 up to a dilution of 1:2 560.

Keywords : ANTIBODIES, MONOCLONAL [diagnostic use]; HERPESVIRUS HOMINIS [isolation & purification]; HY-BRIDOMAS; FLUORESCENT ANTIBODY TECHNIQUE; HYBRIDIZATION; VERO CELLS; MICE, INBRED BALB C.

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