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Revista Cubana de Medicina Tropical
Print version ISSN 0375-0760On-line version ISSN 1561-3054
Abstract
ALBERTI AMADOR, Esteban et al. Proteasa dependiente de cisteína en Trypanosoma cruzi útil en el diagnóstico de la enfermedad de Chagas. Rev Cubana Med Trop [online]. 1998, vol.50, n.1, pp.75-81. ISSN 0375-0760.
An immunoenzymatic system on solid phase for the detection of IgG antibodies in serum from choronic Chagasic patients was standardized. A protease from Trypanosoma cruzi (GP57/51KDa) was used as an antigen. The sensitivity, specificity and predictive values of the procedure were calculated taking into account the results obtained from conventional serology (CS), indirect immunofluorescence (IIF), indirect hemagglutination (IHA) and complement-mediated lysis test (CML) for the detection of anti-Trypanosoma cruzi antibodies in serum. Except one, the positive samples obtained by those techniques were also positive by our method. These resultas show that GP 57/51 is useful in the serodiagnosis of Chagas disease.
Keywords : CHAGAS DISEASE [diagnosis]; CYSTEINE PROTEINASES [diagnostic use]; TRYPANOSOMA CRUZI [immunology]; ANTIGENS, PROTOZOAN [blood]; ENZYME-LINKED IMMUNOSORBENT ASSAY [methods]; SERODIAGNOSIS [methods]; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT [methods]; HEMAGGLUTINATION TESTS [methods].