Abstract

ACOSTA DOMINGUEZ, Armando et al. Respuesta humoral específica en ratones Balb/c inoculados con una librería genómica de expresión de Trypanosoma cruzi. Rev Cubana Med Trop [online]. 1999, vol.51, n.1, pp.20-25. ISSN 0375-0760.

A genomic expresion library of Trypanosoma cruzi (T. cruzi) was made using plasmid pcDNA3 as a vector, with wich male mice from the Balb/c isogenic line were intramuscullary inoculated. It was used a positive control group that was administered soluble antigens of T. cruzi. Other 2 groups received genomic and plasmid DNA, respectively. One group was not immunized. Weeckly blood samples were obtained from all the animals until the fourth week and 2 weeks after reinmunization to study the response of specific antibodies against the microorganism antigens by an indirect immunoenzimatic assay (ELISA). It was observed a significant increase of specific antibodies in the animals reinmunized with 50 mg of the library, as well as in the group immunized with soluble antigens of T. cruzi.

Keywords : DNA, PROTOZOAN [immunology]; GENOME, PROTOZOAN; PROTOZOAN VACCINES [pharmacology]; TRYPANOSOMA CRUZI [genetics]; TRYPANOSOMA CRUZI [immunology]; VACCINES, DNA [pharmacology]; MICE, INBRED BALBC.

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