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Revista Cubana de Medicina Tropical
Print version ISSN 0375-0760On-line version ISSN 1561-3054
Abstract
BISSET, Juan A; RODRIGUEZ, María Magdalena; DIAZ, Cristina and ALAIN SOCA, Lázaro. Caracterización de la resistencia a insecticidas organofosforados, carbamatos y piretroides en Culex quinquefasciatus del Estado de Miranda, Venezuela. Rev Cubana Med Trop [online]. 1999, vol.51, n.2, pp.89-94. ISSN 0375-0760.
Resistance of Culex quinquefasciatus from Miranda, Venezuela to the organophosphate insecticides malathion and chlorpirifos was higher than 30 x whereas resistance to pyrethroids metylpirimifos, fention, cipametrine, deltametrine, permetrine and lambdacyalotrine and to organochlorate DDT was lower than 4x. Resistance mechanisms were analyzed with piperonyl butoxide synergist (multifunction oxidases) and S.S.S. phosphotrithiate tributyl (DEF, esterase inhibitor). Multifunction oxidases did not play a significant role in resistance to organophosphate insecticides and carbamate; however, esterases were only mechanisms of resistance to malathion and chlorpirifos. The only insecticide affected by DEF and PB was cipermetrine. Biochemical tests revealed a very low frequency of the altered acetylcholinesterase mechanism (0,13). Esterase frequencies were high (1). Electrophoresis exposed the B1, A6 and B6 esterase phenotypes.
Keywords : INSECTICIDE RESISTANCE; INSECTICIDES; ORGANO PHOSPHATE; BIOCHEMICAL REACTION; AEDES.