SciELO - Scientific Electronic Library Online

 
vol.51 número3Sistema de vigilancia de eventos adversos consecutivos a la vacunación en la República de CubaHaemophilus influenzae circulantes en Cuba. Caracterización de cepas aisladas de meningitis índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Revista Cubana de Medicina Tropical

versão On-line ISSN 1561-3054

Resumo

MENDIOLA MARTINEZ, Judith; HERNANDEZ ALVAREZ, Hilda  e  FINLAY VILLALVILLA, Carlos M.. Actividad semejante a catepsina L en la hemolinfa de hembras repletas de Boophilus microplus. Rev Cubana Med Trop [online]. 1999, vol.51, n.3, pp. 201-203. ISSN 1561-3054.

The cysteine-proteinase activity present in the hemolymph of females crammed with Boophilus microplus at the second oviposition was proved. The enzime assay was performed using the synthetic N-carbobenzoxi-phenilalanyl-arginil-4-metoxi-betan-naphthylamide substrate, and hydrolisis in both raw ovarim extract and hemolymph were observed. The activity was 100 % inhibited by trans-epoxisuscinyl-L- leucidamide (4-guadinine) butane, an specific inhibitor for cysteine-proteinase. Polyacrylamide and gelatin-gel electrophoresis showed the existance of only one active band in both samples tested with the same electrophoretic sliding (approximately 38 kDa).

Palavras-chave : CATHEPSINS [blood]; HEMOLYMPH.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License