Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
ESPINOSA, Ivette et al. Obtención de un conjugado látex inmunoglobulina para el diagnóstico de Gardnerella vaginalis. Rev Cubana Med Trop [online]. 2000, vol.52, n.2, pp. 101-105. ISSN 1561-3054.
To obtain an anti-Gardnerella vaginalis latex globuline, specific immunosera to reference strain (ATCC 14018) and to a clinical isolation strain pool were produced. These inmunosera were characterized using PAGE-SDS and inmunoblotting where a close antigenic relation between the clinical isolation strains and the reference strain was observed. The latex globuline conjugates obtained from these 2 inmunosera were evaluated in vitro with a resulting level of detection of 10 ufc/mL of Gardnerella vaginalis. These two conjugates were also evaluated in clinical samples and compared with the 6 vaginalis culture and the criteria considered for diagnosing bacterial vaginosis. The anti-strain pool (II) latex globuline conjugate turned out to be more sensitive and specific than anti-G. vaginalis ATCC strain (I) latex globuline.
Keywords : GARDNERELLA VAGINALIS [isolation and purification]; VAGINOSIS, BACTERIAL [diagnosis]; LATEX FIXATION TESTS.