Revista Cubana de Medicina Tropical
versión On-line ISSN 1561-3054
NAVEA LEYVA, Leonor M. et al. Anticuerpos neutralizantes frente a 5 cepas de VIH-1 en macacos inmunizados con el polipéptido multiepitópico TAB9. Rev Cubana Med Trop [online]. 2000, vol.52, n.2, pp. 115-118. ISSN 1561-3054.
Presence of neutralizing antibodies(Nab) to 5 laboratory strains of human immunodeficiency virus type-1 (HIV-1) was studied and compared in the sera of eight macaca (Macaca fascicularis) after the third and fourth immunizations with multi-epitope polypeptide TAB9, emulsified with adjuvant Montanide ISA 720. Four animals were innoculated 1mg and the others 200 µg, 2 animals were used as controls and injected only with adjuvant. Although the presence of neutralizing antibodies to homologous strains of HIV-group group B was confirmed in most animals after immunization through antigen p24 capture ELISA (DAVIH-Agp24, Cuba), no statistically significant differences were found neither in titers caused by antigen concentrations nor in the responses after the third and fourth immunizing dosis. Controls did not develop neutralizing antibodies.
Palabras llave : HIV-1 [immunology]; HIV-1 blood; HIV ANTIBODIES [immunology]; PEPTIDES; EPITOPES; MACACA; IMMUNIZATION; PASSIVE.