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Revista Cubana de Medicina Tropical

versión impresa ISSN 0375-0760versión On-line ISSN 1561-3054

Resumen

ROSSI, Nineth; RIVAS, Ivan; HERNANDEZ, Manuel  y  URDANETA, Haideé. Inmunodiagnóstico de la neurocisticercosis: estudio comparativo de extractos antigénicos de Cysticercus cellulosae y Taenia crassiceps. Rev Cubana Med Trop [online]. 2000, vol.52, n.3, pp.157-164. ISSN 0375-0760.

Different antigenic extracts of Taenia solium and Taenia crassiceps were evaluated in connection with the detection of antibodies in patients with neurocysticercosis aimed at selecting immunorelevant antigens for the diagnosis of neurocysticercosis by means of the immunoenzimatic assay and immunoblotting. The vesicular fluid of T. crassiceps proved to be more sensitive (100%) and specific (86%). On using the immunoblotting technique it was also observed that this extract was the most sensitive and specific. Within the protein profile of the antigen the band of 18 kDa was mostly recognized by the serum and cerebrospinal fluid of patients with neurocysticercosis. The vesicular fluid of T. crassiceps represents an alternative in the optimization of the diagnosis of neurocysticercosis in the serum and cerebrospinal fluid and in the substitution of T. solium antigens due to its high sensitivity and specificity and to its easy obtention under controlled laboratory conditions.

Palabras clave : NEUROCYSTICERCOSIS [immunology]; TAENIASIS; ANTIGENS; HELMINTH; ENZYME-LINKED IMMUNOSORBENT ASSASY [methods]; IMMUNOBLOTTING.

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