Abstract

HERNANDEZ ALVAREZ, Hilda M.; MENDIOLA MARTINEZ, Judith; FERNANDEZ-CALIENES, Aymé  and  VALDEZ, Mario. Identificación de una proteasa neutra en intestino de Boophilus microplus por electroforesis en geles de poliacrilamida copolimerizados con gelatina. Rev Cubana Med Trop [online]. 2000, vol.52, n.3, pp.165-169. ISSN 0375-0760.

A neutral activity of Boophilus microplus in the intestine was identified by electrophoresis in polyacrilamide gel copolymerized with gelatin. The maximum of activity was attained at pH 6.0. The highest specific activity at that pH was obtained with casein substrate. The disappearance of this activity was observed in both substrates after the addition of phenylmethylsulphonyl fluoride in the reaction mixtures. It was very interesting to find out an endopeptidase activity with these characteristics in the intestine, in spite of the fact that the digestive activity in ticks is intracellular at very acid pH, which does not occur in other insects.

Keywords : ELECTROPHORESIS; POLYACRYLA-MIDE GEL [methods]; TICKS [enzimology]; PEPTIDE PEP-TIDOHYDROLASES [analysis]; INTESTINES.

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