Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
ALVAREZ, Elba; RODRIGUEZ, Beatriz; ROSADO, Ileana and GONZALEZ, Marisol. Obtención, caracterización de antígenos y evaluación de la técnica de inmunotransferencia para la detección de candidiasis sistémica. Rev Cubana Med Trop [online]. 2000, vol.52, n.3, pp. 186-190. ISSN 1561-3054.
A culture media containing peptone, glucose, yeast extract and serum was evaluated to promote the development of the mycelium formation in Candida albicans and it was compared with the commercial media Tc 199. No signficant difference was found between them. Somatic antigens characterized by PAGE-SDS were obtained from yeast and mycelial forms. Proteins reported as having high diagnostic value were expressed in both antigens. It was determined by immunotransference that the antigenic proteins predominating in the mycelium formation had molecular weights of 19, 21, 27 and 57 Kda. The immunotransference was standardized for detecting antibodies in patients with systemic candidiasis. 24 positive sera were evaluated by this technique and it was compared with the ELISA technique, which proved to be more sensitive and less specific. The most recognized proteins were those of 136 and 21 Kda and the complex from 42 to 45 Kda.
Keywords : ANTIGENS; ENZIME-LINKED IMMUNO-SORBENT ASSAY [methods]; CANDIDA ALBICANS; CULTURE MEDIA.