Resumo

MENDIOLA, Judith et al. Aislamiento de aspártico-proteinasas en diferentes estadios de desarrollo de la garrapata Boophilus microplus. Rev Cubana Med Trop [online]. 2001, vol.53, n.3, pp. 145-153. ISSN 1561-3054.

The magnitud of tisular expression of aspartic proteinases at juvenile stages was analyzed in adult young Boophilus microplus ticks as well as the effect of one of the most widely used aspartic proteinase inhibitors (pepstatin A) on their development. The isolation and quantification of acid proteolytic activity was performed in samples of parasites with different stages of the digestive process where aspartic-proteinase activity was confirmed and a tendency to increased values of enzymatic activity per miligram of total weight was observed during the development of adults female ticks, with a lowering of this ratio just when they are full up. Adding pepstatin A to the ingesta at 14 and 70 mmol concentrations had no effect on the viability of young female ticks while they were kept in vitro for 24 h, so this rules out the immediate toxic effects of this compound. However, in those experiments where adult female ticks after receiving the various ingestas, are put together with sexually competent male ticks in isolators for 6-7 days, the viability showed different results.

Palavras-chave : ASPARTIC ENDOPEPTIDASES [isolation & purification]; PEPSTATINS; TICK [growth & development]; TICK INFESTATIONS; PROTEASE INHIBITORS.

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