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vol.54 issue1Comportamiento clínico-epidemiológico de la leptospirosis humana en el municipio Los Palacios, provincia Pinar del Río, Cuba author indexsubject indexarticles search
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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054

Abstract

TRUEBA, Gabriel; ZAPATA, Sonia; MADRID, Kleber  and  PENAFIEL, Nicolás. Adaptación de Leptospira interrogans (sensu stricto) al agua dulce. Rev Cubana Med Trop [online]. 2002, vol.54, n.1, pp.11-14. ISSN 0375-0760.

The contact with polluted waters is one of the main risk factors to catch leptospirosis. A study is presented about the adaptation of Leptospira interrograns to nutrient-lacking water media. For this end, leptospires were incubated in distilled water and tampon saline solution for an undetermined period of time. Leptospires kept viable in water for 98 days whereas the incubated ones in tampon saline solution survived 3 weeks only. Protein cellular and external membrane components were analyzed with electrophoresis in acrylamide gel (SDS-PAGE). When OM protein profiles of leptospires kept in water were compared to those OM profiles of cells cultured in ENJA medium, some differences were observed. A 56 kDa protein was present in leptospires kept in water for a week. This protein was identified as GroEL through Western Blot test.

Keywords : LEPTOSPIRA INTERROGANS [isolation & purification]; WATER POLLUTION; RISK FACTORS; CULTURE MEDIA; LEPTOSPIROSIS [epidemiology]; LEPTOSPIROSIS [microbiology]; FRESH WATER.

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