Abstract

GONZALEZ RODRIGUEZ, Andrés; BATISTA SANTIESTEBAN, Niurka; VALDES ABREU, Yolanda  and  GONZALEZ GONZALEZ, Marta. Crecimiento, virulencia y antigenicidad de Leptospira interrogans serovar mozdok en medio EMJH modificado. Rev Cubana Med Trop [online]. 2002, vol.54, n.1, pp.32-36. ISSN 0375-0760.

The effect of higher Tween 80 concentrations in EMJH synthetic medium on the growth, virulence and antigenecity of Leptospira interrogans serovar mozdok was evaluated for increasing the performances and making a full use of the detoxifying capacity of bovine serum albumin. The growth was spectrophotographically evaluated by the analysis of the bacterial growth kinetics; the obtained biomass performance and the consumption of the carbon source. The virulence was estimated in Syrian Hamster model whereas antigenecity was determined through the microagglutination technique in rabbit’s polyclonal antiserum. Under controlled culture conditions, the increase of Tween 80 concentration up to 3,25 mg/ml brought about an acceleration in bacterial metabolism that managed to double cell performances with a full consumption of the carbon source, without affecting virulence and antigenecity for a number of successive subcultures.

Keywords : LEPTOSPIRA INTERROGANS [isolation & purification]; LEPTOSPIRA INTERROGANS [growth & development]; VIRULENCE; CULTURE MEDIA; LEPTOSPIROSIS [diagnosis]; AGGLUTINATION TESTS; SERUM ALBUMIN; BOVINE.

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