Revista Cubana de Medicina Tropical
versão On-line ISSN 1561-3054
GONZALEZ SOSA, Nibaldo L. et al. Legionella pneumophila: extracción de la proteína principal de la membrana externa (p 29). Rev Cubana Med Trop [online]. 2002, vol.54, n.2, pp. 91-95. ISSN 1561-3054.
Legionella pneumophila is an important opportunistic pulmonary pathogen that causes Legionaires´diseases. In the outer membrane of the bacterium, there is a protein with molecular weight of 29 kd(p 29), which is not only an specific antigen of the species but also the major outer membrane protein; it may also be a factor of pathogenecity in the bacterium. The main objective of this paper was the extraction and purification of this protein by treating the outer membrane with chemical agents. A p 29-rich semipurified antigen extract was obtained that can be used as an important antigen for the development of immunodiagnostic techniques in future studies and also for obtaining a monoclonal antibody to be used for the same purpose.
Palavras-chave : BACTERIAL OUTER MEMBRANE PROTEINS [isolation & purification]; LEGIONELLA PNEUMOPHILA; ANTIGENS, BACTERIAL; IMMUNOLOGIC TESTS; ANTIBODIES, MONOCLONAL.