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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054

Abstract

ROSALES MANRIQUE, Osbert; URDANETA ROMERO, Haideé; HERNANDEZ BARRIOS, Manuel  and  MUNOZ, José Francisco. Evaluación de la actividad de polimorfonucleares neutrófilos frente a antígenos de cepas patógenas de Entamoeba histolytica. Rev Cubana Med Trop [online]. 2002, vol.54, n.2, pp.96-100. ISSN 0375-0760.

This paper evaluates the production of superoxide as an indicator of the activity of neutrophils from healthy individuals, stimulated with Entamoeaba histolytica membrane antigens, chemical stimulators and antigens from other parasites. The kinetic nitroblue tetrazolium reduction test was used. No significant differences were observed in the control group as to superoxide production by neutrophils primed with Entamoeba histolytica antigens or Cisticercus cellulosae, Toxoplasma gondii and Giardia lamblia antigens. These results allow us to conclude that Entamoeba histolytica membrane antigens do not induce superoxide production by neutrophils, which contradicts previous statements on suppresed activity of neutrophils after they get in contact with Entamoeaba histolytica antigens.

Keywords : ENTAMOEBA HISTOLYTICA [isolation & purification]; NEUTROPHILS [immunology]; SUPEROXIDES.

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