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Revista Cubana de Medicina Tropical
Print version ISSN 0375-0760On-line version ISSN 1561-3054
Abstract
CAPO DE PAZ, Virginia et al. Diagnóstico de coccidias y microsporas en muestras de heces diarreicas de pacientes cubanos seropositivos al VIH: primer reporte de microsporas en Cuba. Rev Cubana Med Trop [online]. 2003, vol.55, n.1, pp.14-18. ISSN 0375-0760.
The numerous specimens of liquid diarrheas received at the clinical parasitology laboratory of "Pedro Kouri" Institute of Tropical Medicine were studied searching for the presence of pathogenic agents of Coccidium subclass and of the microspore family. The conditions to diagnose microsporidia were created on July 13, 2000, and the method was incorporated to the battery of diagnostic techniques used in the laboratory. A cut-off was made after 4 months to analyze the positivity found. A frequency distribution study was conducted and it was observed that of the 170 studied samples, 51 proved to be positive for some protozoon, which represents 30 % of positivity. The most frequently found species were Cryptosporidium parvum and microsporidium. Association of various species in a same sample was found in 13.1 % of the positive samples. The most frequent associations were: Cryptosporidium parvum and Cyclospora cayetanensis, Cryptosporidium parvum and microsporidium, and Cyclospora cayetanensis and microsporidium, each with 2 patients. Association of 3 different species in a same sample was also found. All the individuals in the series were HIV seropositive and the most affected had less than 200 TCD4+ lymphocytes/mL. This was the first report of microsporidia in feces in Cuba. It was possible after applying and interpreting the technique previously described to identify this phylum. As there is a trend towards the increase of cases with HIV/AIDS infection, it is necessary that the laboratories have the indispendable reagents to perform the modified Didiers trichromic staining and that the professionals and technicians of the parasitology laboratories of the country be trained in the procedures for identifying and recognizing these opportunistic protozoa.
Keywords : TUBERCULOSIS [mortality]; TUBER-CULOSIS [history]; TUBERCULOSIS PULMONARY [mortality]; TUBERCULOSIS PULMONARY [history]; MORTALITY RATE; CUBA.
